Autophagy benefits the replication of Newcastle disease virus in chicken cells and tissues.
Identifieur interne : 001036 ( Main/Exploration ); précédent : 001035; suivant : 001037Autophagy benefits the replication of Newcastle disease virus in chicken cells and tissues.
Auteurs : Yingjie Sun [République populaire de Chine] ; Shengqing Yu ; Na Ding ; Chunchun Meng ; Songshu Meng ; Shilei Zhang ; Yuan Zhan ; Xusheng Qiu ; Lei Tan ; Hongjun Chen ; Cuiping Song ; Chan DingSource :
- Journal of virology [ 1098-5514 ] ; 2014.
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Abstract
Newcastle disease virus (NDV) is an important avian pathogen. We previously reported that NDV triggers autophagy in U251 glioma cells, resulting in enhanced virus replication. In this study, we investigated whether NDV triggers autophagy in chicken cells and tissues to enhance virus replication. We demonstrated that NDV infection induced steady-state autophagy in chicken-derived DF-1 cells and in primary chicken embryo fibroblast (CEF) cells, evident through increased double- or single-membrane vesicles, the accumulation of green fluorescent protein (GFP)-LC3 dots, and the conversion of LC3-I to LC3-II. In addition, we measured autophagic flux by monitoring p62/SQSTM1 degradation, LC3-II turnover, and GFP-LC3 lysosomal delivery and proteolysis, to confirm that NDV infection induced the complete autophagic process. Inhibition of autophagy by pharmacological inhibitors and RNA interference reduced virus replication, indicating an important role for autophagy in NDV infection. Furthermore, we conducted in vivo experiments and observed the conversion of LC3-I to LC3-II in heart, liver, spleen, lung, and kidney of NDV-infected chickens. Regulation of the induction of autophagy with wortmannin, chloroquine, or starvation treatment affects NDV production and pathogenesis in tissues of both lung and intestine; however, treatment with rapamycin, an autophagy inducer of mammalian cells, showed no detectable changes in chicken cells and tissues. Moreover, administration of the autophagy inhibitor wortmannin increased the survival rate of NDV-infected chickens. Our studies provide strong evidence that NDV infection induces autophagy which benefits NDV replication in chicken cells and tissues.
DOI: 10.1128/JVI.01849-13
PubMed: 24173218
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first="Na" last="Ding">Na Ding</name></author><author><name sortKey="Meng, Chunchun" sort="Meng, Chunchun" uniqKey="Meng C" first="Chunchun" last="Meng">Chunchun Meng</name></author><author><name sortKey="Meng, Songshu" sort="Meng, Songshu" uniqKey="Meng S" first="Songshu" last="Meng">Songshu Meng</name></author><author><name sortKey="Zhang, Shilei" sort="Zhang, Shilei" uniqKey="Zhang S" first="Shilei" last="Zhang">Shilei Zhang</name></author><author><name sortKey="Zhan, Yuan" sort="Zhan, Yuan" uniqKey="Zhan Y" first="Yuan" last="Zhan">Yuan Zhan</name></author><author><name sortKey="Qiu, Xusheng" sort="Qiu, Xusheng" uniqKey="Qiu X" first="Xusheng" last="Qiu">Xusheng Qiu</name></author><author><name sortKey="Tan, Lei" sort="Tan, Lei" uniqKey="Tan L" first="Lei" last="Tan">Lei Tan</name></author><author><name sortKey="Chen, Hongjun" sort="Chen, Hongjun" uniqKey="Chen H" first="Hongjun" last="Chen">Hongjun Chen</name></author><author><name sortKey="Song, Cuiping" sort="Song, Cuiping" 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Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Shengqing" sort="Yu, Shengqing" uniqKey="Yu S" first="Shengqing" last="Yu">Shengqing Yu</name></author><author><name sortKey="Ding, Na" sort="Ding, Na" uniqKey="Ding N" first="Na" last="Ding">Na Ding</name></author><author><name sortKey="Meng, Chunchun" sort="Meng, Chunchun" uniqKey="Meng C" first="Chunchun" last="Meng">Chunchun Meng</name></author><author><name sortKey="Meng, Songshu" sort="Meng, Songshu" uniqKey="Meng S" first="Songshu" last="Meng">Songshu Meng</name></author><author><name sortKey="Zhang, Shilei" sort="Zhang, Shilei" uniqKey="Zhang S" first="Shilei" last="Zhang">Shilei Zhang</name></author><author><name sortKey="Zhan, Yuan" sort="Zhan, Yuan" uniqKey="Zhan Y" first="Yuan" last="Zhan">Yuan Zhan</name></author><author><name sortKey="Qiu, Xusheng" sort="Qiu, Xusheng" uniqKey="Qiu X" first="Xusheng" last="Qiu">Xusheng Qiu</name></author><author><name sortKey="Tan, Lei" sort="Tan, Lei" uniqKey="Tan L" first="Lei" last="Tan">Lei Tan</name></author><author><name sortKey="Chen, Hongjun" sort="Chen, Hongjun" uniqKey="Chen H" first="Hongjun" last="Chen">Hongjun Chen</name></author><author><name sortKey="Song, Cuiping" sort="Song, Cuiping" uniqKey="Song C" first="Cuiping" last="Song">Cuiping Song</name></author><author><name sortKey="Ding, Chan" sort="Ding, Chan" uniqKey="Ding C" first="Chan" last="Ding">Chan Ding</name></author></analytic><series><title level="j">Journal of virology</title><idno type="eISSN">1098-5514</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Autophagy</term><term>Cells, Cultured</term><term>Chick Embryo</term><term>Microscopy, Electron, Transmission</term><term>Newcastle disease virus (physiology)</term><term>RNA Interference</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Cellules cultivées</term><term>Embryon de poulet</term><term>Interférence par ARN</term><term>Microscopie électronique à transmission</term><term>Réplication virale</term><term>Virus de la maladie de Newcastle (physiologie)</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la maladie de Newcastle</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Newcastle disease virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Autophagy</term><term>Cells, Cultured</term><term>Chick Embryo</term><term>Microscopy, Electron, Transmission</term><term>RNA Interference</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Cellules cultivées</term><term>Embryon de poulet</term><term>Interférence par ARN</term><term>Microscopie électronique à transmission</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Newcastle disease virus (NDV) is an important avian pathogen. We previously reported that NDV triggers autophagy in U251 glioma cells, resulting in enhanced virus replication. In this study, we investigated whether NDV triggers autophagy in chicken cells and tissues to enhance virus replication. We demonstrated that NDV infection induced steady-state autophagy in chicken-derived DF-1 cells and in primary chicken embryo fibroblast (CEF) cells, evident through increased double- or single-membrane vesicles, the accumulation of green fluorescent protein (GFP)-LC3 dots, and the conversion of LC3-I to LC3-II. In addition, we measured autophagic flux by monitoring p62/SQSTM1 degradation, LC3-II turnover, and GFP-LC3 lysosomal delivery and proteolysis, to confirm that NDV infection induced the complete autophagic process. Inhibition of autophagy by pharmacological inhibitors and RNA interference reduced virus replication, indicating an important role for autophagy in NDV infection. Furthermore, we conducted in vivo experiments and observed the conversion of LC3-I to LC3-II in heart, liver, spleen, lung, and kidney of NDV-infected chickens. Regulation of the induction of autophagy with wortmannin, chloroquine, or starvation treatment affects NDV production and pathogenesis in tissues of both lung and intestine; however, treatment with rapamycin, an autophagy inducer of mammalian cells, showed no detectable changes in chicken cells and tissues. Moreover, administration of the autophagy inhibitor wortmannin increased the survival rate of NDV-infected chickens. Our studies provide strong evidence that NDV infection induces autophagy which benefits NDV replication in chicken cells and tissues. </div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Hongjun" sort="Chen, Hongjun" uniqKey="Chen H" first="Hongjun" last="Chen">Hongjun Chen</name><name sortKey="Ding, Chan" sort="Ding, Chan" uniqKey="Ding C" first="Chan" last="Ding">Chan Ding</name><name sortKey="Ding, Na" sort="Ding, Na" uniqKey="Ding N" first="Na" last="Ding">Na Ding</name><name sortKey="Meng, Chunchun" sort="Meng, Chunchun" uniqKey="Meng C" first="Chunchun" last="Meng">Chunchun Meng</name><name sortKey="Meng, Songshu" sort="Meng, Songshu" uniqKey="Meng S" first="Songshu" last="Meng">Songshu Meng</name><name sortKey="Qiu, Xusheng" sort="Qiu, Xusheng" uniqKey="Qiu X" first="Xusheng" last="Qiu">Xusheng Qiu</name><name sortKey="Song, Cuiping" sort="Song, Cuiping" uniqKey="Song C" first="Cuiping" last="Song">Cuiping Song</name><name sortKey="Tan, Lei" sort="Tan, Lei" uniqKey="Tan L" first="Lei" last="Tan">Lei Tan</name><name sortKey="Yu, Shengqing" sort="Yu, Shengqing" uniqKey="Yu S" first="Shengqing" last="Yu">Shengqing Yu</name><name sortKey="Zhan, Yuan" sort="Zhan, Yuan" uniqKey="Zhan Y" first="Yuan" last="Zhan">Yuan Zhan</name><name sortKey="Zhang, Shilei" sort="Zhang, Shilei" uniqKey="Zhang S" first="Shilei" last="Zhang">Shilei Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Sun, Yingjie" sort="Sun, Yingjie" uniqKey="Sun Y" first="Yingjie" last="Sun">Yingjie Sun</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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